Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat.
Identifieur interne : 001279 ( Main/Exploration ); précédent : 001278; suivant : 001280Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat.
Auteurs : Jinju Li [République populaire de Chine] ; Guangxu Zhang [République populaire de Chine] ; Dalong Cheng [République populaire de Chine] ; Hua Ren [République populaire de Chine] ; Min Qian [République populaire de Chine] ; Bing Du [République populaire de Chine]Source :
- Gene [ 1879-0038 ] ; 2015.
Descripteurs français
- KwdFr :
- Alignement de séquences, Analyse de séquence d'ADN, Animaux, Antiviraux (pharmacologie), Chiroptera (génétique), Chiroptera (immunologie), Données de séquences moléculaires, Facteur de transcription STAT-1 (génétique), Humains, Immunité innée (immunologie), Interféron bêta (biosynthèse), Interféron bêta (génétique), Lignée cellulaire, Poly I-C (pharmacologie), Similitude de séquences, Souris, Stomatite vésiculeuse (immunologie), Séquence d'acides aminés, Séquence nucléotidique, Ubiquitin-protein ligases (génétique), Virus de la stomatite vésiculeuse de type Indiana (immunologie).
- MESH :
- biosynthèse : Interféron bêta.
- génétique : Chiroptera, Facteur de transcription STAT-1, Interféron bêta, Ubiquitin-protein ligases.
- immunologie : Chiroptera, Immunité innée, Stomatite vésiculeuse, Virus de la stomatite vésiculeuse de type Indiana.
- pharmacologie : Antiviraux, Poly I-C.
- Alignement de séquences, Analyse de séquence d'ADN, Animaux, Données de séquences moléculaires, Humains, Lignée cellulaire, Similitude de séquences, Souris, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antiviral Agents (pharmacology), Base Sequence, Cell Line, Chiroptera (genetics), Chiroptera (immunology), Humans, Immunity, Innate (immunology), Interferon-beta (biosynthesis), Interferon-beta (genetics), Mice, Molecular Sequence Data, Poly I-C (pharmacology), STAT1 Transcription Factor (genetics), Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Ubiquitin-Protein Ligases (genetics), Vesicular Stomatitis (immunology), Vesicular stomatitis Indiana virus (immunology).
- MESH :
- chemical , biosynthesis : Interferon-beta.
- chemical , genetics : Interferon-beta, STAT1 Transcription Factor, Ubiquitin-Protein Ligases.
- chemical , pharmacology : Antiviral Agents, Poly I-C.
- genetics : Chiroptera.
- immunology : Chiroptera, Immunity, Innate, Vesicular Stomatitis, Vesicular stomatitis Indiana virus.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Humans, Mice, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology.
Abstract
Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection.
DOI: 10.1016/j.gene.2015.02.013
PubMed: 25680291
Affiliations:
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Sciences, East China Normal University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Guangxu" sort="Zhang, Guangxu" uniqKey="Zhang G" first="Guangxu" last="Zhang">Guangxu Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Dalong" sort="Cheng, Dalong" uniqKey="Cheng D" first="Dalong" last="Cheng">Dalong Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Hua" sort="Ren, Hua" uniqKey="Ren H" first="Hua" last="Ren">Hua Ren</name><affiliation wicri:level="1"><nlm:affiliation>Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, 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University, Shanghai, China. Electronic address: bdu@bio.ecnu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author></analytic><series><title level="j">Gene</title><idno type="eISSN">1879-0038</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antiviral Agents (pharmacology)</term><term>Base Sequence</term><term>Cell Line</term><term>Chiroptera (genetics)</term><term>Chiroptera (immunology)</term><term>Humans</term><term>Immunity, Innate (immunology)</term><term>Interferon-beta (biosynthesis)</term><term>Interferon-beta (genetics)</term><term>Mice</term><term>Molecular Sequence Data</term><term>Poly I-C (pharmacology)</term><term>STAT1 Transcription Factor (genetics)</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology</term><term>Ubiquitin-Protein Ligases (genetics)</term><term>Vesicular Stomatitis (immunology)</term><term>Vesicular stomatitis Indiana virus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Antiviraux (pharmacologie)</term><term>Chiroptera (génétique)</term><term>Chiroptera (immunologie)</term><term>Données de séquences moléculaires</term><term>Facteur de transcription STAT-1 (génétique)</term><term>Humains</term><term>Immunité innée (immunologie)</term><term>Interféron bêta (biosynthèse)</term><term>Interféron bêta (génétique)</term><term>Lignée cellulaire</term><term>Poly I-C (pharmacologie)</term><term>Similitude de séquences</term><term>Souris</term><term>Stomatite vésiculeuse (immunologie)</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Ubiquitin-protein ligases (génétique)</term><term>Virus de la stomatite vésiculeuse de type Indiana (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-beta</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interferon-beta</term><term>STAT1 Transcription Factor</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Poly I-C</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron bêta</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chiroptera</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chiroptera</term><term>Facteur de transcription STAT-1</term><term>Interféron bêta</term><term>Ubiquitin-protein ligases</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Chiroptera</term><term>Immunité innée</term><term>Stomatite vésiculeuse</term><term>Virus de la stomatite vésiculeuse de type Indiana</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Chiroptera</term><term>Immunity, Innate</term><term>Vesicular Stomatitis</term><term>Vesicular stomatitis Indiana virus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Poly I-C</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Humans</term><term>Mice</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Similitude de séquences</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection. </div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Jinju" sort="Li, Jinju" uniqKey="Li J" first="Jinju" last="Li">Jinju Li</name></noRegion><name sortKey="Cheng, Dalong" sort="Cheng, Dalong" uniqKey="Cheng D" first="Dalong" last="Cheng">Dalong Cheng</name><name sortKey="Du, Bing" sort="Du, Bing" uniqKey="Du B" first="Bing" last="Du">Bing Du</name><name sortKey="Qian, Min" sort="Qian, Min" uniqKey="Qian M" first="Min" last="Qian">Min Qian</name><name sortKey="Ren, Hua" sort="Ren, Hua" uniqKey="Ren H" first="Hua" last="Ren">Hua Ren</name><name sortKey="Zhang, Guangxu" sort="Zhang, Guangxu" uniqKey="Zhang G" first="Guangxu" last="Zhang">Guangxu Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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